On Apr 9, 10:13 am, Duncan Clark <blackh...@abuse.plus.com> wrote: > Historians believe that in newspost > <9de680cc-d2fe-478c-ba5d-bfbc2b53b...@u31g2000yqb.googlegroups.com> on > Thu, 8 Apr 2010, LeboMad <lmaduba...@gmail.com> penned the following > literary masterpiece: > > >2mM dNTP > > 0.2mM surely? 2mM would chelate out all the Mg and it would never work. > > I assume each PCR product amplifies fine in a non-multiplex and only > when you multiplex there is a problem? > > Primer levels used? > > Duncan > -- > I love deadlines. I especially like the whooshing noise they make as > they go flying by. > > Duncan Clark > GeneSys Ltd.
Hey Duncan thanks for the response. You're right it's 0.2mM it was just a typing error. The problem is not really the multiplexing because it worked in the past perfectly I could attach a gel or two if I could. Primer level you referring to? kind regards Lebo _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
