hello everyone, i am encountring problem in Methylation Sensitive Amplified Polymorphism . i restricted DNA with 2 isoshizomers *i.e* MspI and HpaII separately along with EcoRI as the common enzyme. reestriction is OK. but adaptors are not being ligated successfully to the restricted fragments. what i see is a band ahead of the dye which i probably think is the unligated adaptors. and if u cud plz suggest a protocol for the preparation of adaptors
-- Parivartan Vishal School of Biotechnology Univ. of Jammu Jammu INDIA _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
