oops! RNA probes, my bad..... I talked about *DNA probes* for detecting RNA. Apologies for the misunderstanding
P. > Hi, > > > > I have been using ã-[32P]ATP to radiolable my RNA, but I can not find the > > reason why we only use ã-[32P]ATP to radiolable RNA, why do not use other > > ã-[32P]NTPs? > > > > Thanks, > > > > Ruiying > > > > Hello > > > We make RNA probes using PCR with [32P]dCTP in the mix for labelling. So > far it has worked great, always. This is actually the only dNTP we use for > the labelling, but I don't discard its posible to use others. > > P. > > > ------------------------------ > > > > > > > > > ------------------------------ > > _______________________________________________ > Methods mailing list > Methods@net.bio.net > http://www.bio.net/biomail/listinfo/methods > > End of Methods Digest, Vol 59, Issue 11 > *************************************** > _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
