Biocompatible buffer that fits to the electroporation buffer? There was either just no other suitable compound available when the method was developed? A competitor has a patent on the same method, but is using PBS? Just some thoughts
Wo > I need to transform S aureus strain with a plasmid of my interest. > > In the biorad gene pulser protocol booklet, they mention using SMMP > (sucrose, MgCl2, maleic acid and penassay broth) for reviving the s aureus > after the electroporation step. > > I was wondering what is the role of maleic acid in this preparation. > > Can it be substituted with anything else? > > -- > -- > Yoginee Budhkar > Contact: +91 9223355677 _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
