This page recommends 0.012% bromophenol blue in the upper stacking gel for this purpose, and has photos.
http://bitesizebio.com/2010/03/22/sds-page-the-easy-way-to-find-the-wells/ ~ Ben T. From: "Iraz Toprak Aydin" <iraz.ay...@epfl.ch> Date: May 18, 2010 3:37:48 AM PDT To: <meth...@magpie.bio.indiana.edu> Subject: how to color the stacking gel? Hello! I have seen some SDS PAGE gels with red or orange stacking part that makes the wells visible. They use phenol red for the red stacking gel. But I couldn't find the concentration of phenol red they use for this purpose. Does anybody have any experience in this? Thanks in advance! Iraz Toprak Aydin EPFL SV ISREC, Station 19 Batiment SV, SV 2540 CH-1015 Lausanne Switzerland Tel: +41 21 693 07 36 e-mail: <mailto:iraz.ay...@epfl.ch> iraz.ay...@epfl.ch _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
