Dear Bionetters, I am trying to amplify the 28s rRNA region of a fungus. For this i already isolated the genomic DNA and amplified it with ITS-5 and ITS-4 primers. I got a PCR product of length approx. 700bp. However this amplified product covers the region between 18s-5.8s-28s. Could anyone please suggest me the universal primers that specifically amplify the 28s region alone?
I also used ITS-1 and ITS-4 primers for amplification, however no amplification was observed. Please let me know the reason for this. Thanking you in advance. Thanks, Adroit _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
