Hello All I'm performing transport assays using 3H-labeled ATP and our scintilation counter is so old the manuals are lost and the standards used for it are as well. Nevertheless the thing works.
My question is regarding the calculation of dpms. As far as I know the efficiency of the apparatus to measure the isotope is what defines the relationship between cpms and dpms. I managed to find some 3H unquenched standards from 1966. The date should not be a problem as the decay can be easily calculated. The problem is the scintillation liquid they are in. I have no idea what the cocktail might be, although its must be a mix of toluene, which is what was used in the old days. We use a biodegradable-scintillation liquid. If I use these standards in this solution, can they be compared to the counts I obtain using the other scintillation liquid? Should I purchase a standard in the same liquid as I use? Also the vials are different. The standards are in a transparent vial and we use plastic opaque vials for our measurements. Also an issue?? Suggestions appreciated... thanks Priscila _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
