Dear all,
I'm trying to make 6.3kb reverse transcript from mRNA. I tried
both poly dT primer as well as gene specific primers for reverse
transcription. But I didn't get 6.3kb product. So tried to amplify 'C'
terminal half (3.1kb). But I didn't get that also. I have used Superscript
II fro reverse transcription and Phusion polymerase for PCR. If anybody have
any suggestion please give me.
Thanks in advance.
Regards,
B.Ramachandran.
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