Dear all, I have done SDM using Quick Change method by Pfu turbo to insert 72 bases in a gene cloned in pBAD24 for complementation. Now I got to know that I should have done this SDM in some other plasmid and then sub clone in pBAD24. Therefore now I have to again sub clone my mutated genes to new pBAD24.
My question is that is it necessary? Is any published work there where people have used direct SDM pBAD24 plasmid for complementation? Thanks, Arpita _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
