Historians believe that in newspost
<mailman.15.1285213032.3712.meth...@net.bio.net> on Wed, 22 Sep 2010,
Eric Ma <ericmajingl...@gmail.com> penned the following literary
masterpiece:
I'm looking to lyse E. coli cells in order to extract total protein from it
for downstream processing and testing. One idea that came up was to use
sonication so that we don't damage the protein - is this recommended? Also,
if it is, do you have any recommendations on where to find protocols for
this?
Works fine but is tedious for multiple samples. 30secs on a 3mm probe
with 20-50W power in a 1.5ml flip top eppendorf with say 1ml in it. Keep
it cold - say sitting in a small glass bottle filled with iced water.
Sonicate, take out into iced water, do rest of samples and then go back
and give them all a 2nd 30sec go. Microfuge, assay or gel. Done it for
30 years screening for activity. Idiot proof providing one doesn't let
the sampe, get hot so test with your probe and power etc.
Duncan
--
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
Duncan Clark
GeneSys Ltd.
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