On 26/09/2010 02:13, DK wrote:
In article<8g7dvnfjc...@mid.individual.net>, Peter Ellis<pj...@cam.ac.uk>
wrote:
>>
A consideration I hadn't explained is that I'm distinguishing between an
X gene and an autosomal relative, so a peptide antibody was necessary to
direct it to regions of greatest divergence between the two. As for the
ease or otherwise of expressing proteins in E. coli, that's kind of the
problem here!
Well, probably not for all of the protein's fragments?
True, true, though at some point the distinction between "protein
fragment" and "peptide" starts to become a little blurred! I'll bear
this in mind when making antibodies in future. The other project I'm
working on involves distinguishing between three protein families with
>=90% amino acid identity.
Good luck doing that without peptide ABs... but that's a whole different
story. Not a new story if I'm honest, if you do a search on this
newsgroup, it was a couple of years ago that I first started noticing
I'd ended up with a particularly recalcitrant set of target genes.
Peter
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