This protocol ought to do. Big advantage: No DSBs or nicks due to ultraviolet. I would be interested to hear if there is any connection to performance on a spin column, though. If someone already using this protocol could do a quick side-by-side comparison and let us know on this list, I'd be very grateful!
---------- Forwarded message ---------- Try this paper. It suggests using Crystal Violet alone or in combination with Methyl orange, with visualisation on a standard artist's light-box. On 7 November 2010 06:49, gholamreza ahmadian <gholamrezaahmad...@yahoo.ca>wrote: > Dear Colleagues > could I have the protocol for vsualisation of DNA using Crystal Violet in > the > gel? > > thanks > G.A > > > > _______________________________________________ > Methods mailing list > Methods@net.bio.net > http://www.bio.net/biomail/listinfo/methods
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