On Nov 26, 9:36 am, "Haviland, David L" <[email protected]> wrote: > As I run a flow core, I also use just plain old 0.9% saline as well, but... I > know of other flow cytometry centers that use DI as sheath. I B.S. you > not... For the short duration of laminar flow, the central stream of sample > fluid (whatever your cells are in) is centered within the DI sheath. No > one really cares what happens to the cells once past the interrogation point, > let alone the waste line. DI also tends to keep the machines cleaner and a > lot less crystallization around the SIP tube. > > Pointed question: Have I made the switch? Not yet. I have about 60L of > 10X sheath to use up, but a colleague across the way uses DI, and I'm going > to switch after that 60L is gone. > > David Haviland, PhD > UTHSC- Houston Stem Cell Flow Center. > > ________________________________________ > From: [email protected] > [[email protected]] On Behalf Of DK > [[email protected]] > Sent: Wednesday, November 24, 2010 19:11 > To: [email protected] > Subject: Re: does anybody have a recipe for flow cytometry sheath fluid? > > In article > <[email protected]>, Mary > <[email protected]> wrote: > > >Hi All, > >> the liquid stream (sheath fluid), which carries and aligns the cells > >> so that they pass single file through the light beam for sensing in > >> flow cytometers does anybody knowhow to make a DIY sheath fluid? I > >> thought maybe using 0.9% saline solution would work...amy thoughts? > >> regards > > Pretty much. A simple PBS is what most everyone uses. E.g., here > is 1X composition:http://www.biosure.com/rpages/solutions.htm > > Make it 10X and filter through 0.2 micron and it won't go bad even > when non-sterile and without preservatives. > > DK > _______________________________________________ > Methods mailing list > [email protected]http://www.bio.net/biomail/listinfo/methods
David why do I need to filter sterilise the PBS? _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
