In article <[email protected]>, [email protected] says... > > Hello All, > > We have Biorad mini protean unit for vertical electrophoresis. > > I was thinking of getting same dimension glasses molded from a glassmith > near by. But I had to clear only one doubt. Is this glass made of or coated > with any special material to make it inert towards SDS PAGE ingredients?
No, standard glass is just fine. However, you may wish to silanise them with dichlorodimethylsilane (RepelCote, RepelSilane). That results in a 1 molecule thick layer of silicone on the glass, that prevents gels from sticking to the plates when you open the cassettes. In a fume cupboard, just briefly dip the plates in the solution, air-dry and rinse with water. When gels start sticking again after a couple of uses, just repeat the process. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
