On 29 Mrz., 22:45, [email protected] (DK) wrote: > In article <[email protected]>, > [email protected] wrote: > >Dear all, Hi DK,
would Laundry Detergent also work as replacement for proteinase K in mouse tail biopsy genotyping? Or does it also contain DNAse? Just curious. Probably saving some bucks on proteinase K while risking a transgenic mouse project wouldn't make much sense... Wo > > >Can anyone suggest a goof recipe for a lysis buffer that efficiently lyses > >mouse liver and suitable for use in quantification of 55Fe by scintilation > >counting? > > What's the purpose of the lysis? If you just need to get as much stuff > as possible into solution then I'd suggest using 1-5% laundry detergent. > That will really do it, particularly with heating. And very compatible with > most scintillation cocktails. > > If, however, you need to keep somehting intact/functional then it > really depends upon what that something is. > > DK _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
