In article <[email protected]>, [email protected] says... > > Hello everybody, > > I have a question concerning a plasma membrane protein, which have been > expected using different proteomic software to be localized on the plasma > membrane. > > After tagging with gfp it looks to be localized in more than 80% on vesicle > structures which we assumed to be vacuoles. > > Is it possible to be a bifunctional protein such as transporter on plasma > membrane and vacuole? or that could be an artifact of gfp fusion process? > > Is there any programs or software can discriminate between both > compartment's proteins?
Software can make predictions, but those have to be checked by experiment. Homogenisation of the cells and fractionisation on a sucrose gradient in an ultracentrifuge would be a classical way of doing it, or immunofluorescence microscopy. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
