Dear Sudheer, in that case, you may need to remove the copper before the electrophoresis. You might either try gel filtration (keywords: Sephadex-g25 / PD10 / NAP10) or maybe ammonium sulfate precipitation.
Just out of curiosity: can you share/explain us the protocol? Is it something like Click Chemistry (CuBr mediated formation of triazoles by combining azides with ethines or tetrazoles by using nitriles instead?) or the earlier suspected formation of disulfides of proteins which are close to each other? Another possible explanation of the yellow precipitate comes into my mind: A bit analog to what happens in the BCA test: Copper complexes free amino groups (lysines) and when, heating, some chemistry happens that is reducing the copper. - Keld, are you listening by chance? Would you like to comment? Wolfgang On Apr 20, 7:13 pm, sudheer babu <[email protected]> wrote: > Hi > thank you for all replies. I am doing Crosslinking experiment by the way I > dissolved cucle in milli Q water. > > Sudheer Babu.S > ITC student > Institute of Biochemistry > Biological Research Center > Szeged,Hungary. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
