Also just curious: How did the 72degC dogma for the extension step evolve and come into effect? Only optimum for processing speed?
/Wo >> Just curious: why 65C? 5 min total extension for 3 kbp or per every kbp? > It is 65C for 5min total ext time and betaine is critical. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
