Does anyone know of a method for removing proteoglycan contaminants from total RNA extracts? I work with marine invertebrates (corals) and certain species are difficult due to RNA that cannot be successfully manipulated downstream (i.e. labeling, RT, etc.). Other research have suggested that endogenous proteoglycans interfere with enzymes that bind RNA. I use the TRIzol method of extraction and the Qiagen MinElute kit for purifications. I am getting good quality RNA based on nanodrop spectrophotometry (260:280 @ 2.0) and Agilent Bioanalyzer analysis (clean gels, RIN @ 8-9). My 260:230's could be better and tend to be all over the place, but have gotten up to 1.9. I would appreciate any suggestions.
Thank you. Sara Edge, Ph.D. Marine Ecosystem Health Harbor Branch Oceanographic Institute at FAU 5600 US-1 North, Fort Pierce, FL 34946 772-242-2261 [email protected] _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
