There are large number of programs to check primers on the web. There were
great algorithms around the web long before programs like amplify. I would use
Netprimer to check out primers. But also search the site www.expasy.ch for
more free online tools.
Jay
-----Original Message-----
From: [email protected]
[mailto:[email protected]] On Behalf Of Jones, Michael D
Sent: Tuesday, August 09, 2011 7:51 AM
To: [email protected]
Subject: Amplify Software Program and Mac OS X Lion
Hi
Not sure if this is the right place, but here goes.
I use Bill Engels "Amplify" program (works only on a Mac OS X, up to 10.6.8).
It is imho a great little program for checking out primers for use in PCR.
However, it is written for a PowerPC Mac, and will not work with the new Mac
Lion OS.
Anybody know if it will be written to work with Lion, or know of an alternative?
Amplify is not a program designed to 'produce' primer sequences for you, but in
silico mimics a PCR reaction. It checks if there are alternative binding sites
for your primers within a target region, not just perfect matches, but regions
with mismatches, etc.
I never order an oligonucleotide for PCR until I'm happy that it produces a
single priming site with Amplify.
Thanks
Mick
MRC Clinical Sciences Centre
Imperial College London
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