what is the concentration of formamide and 1,5 Pentanediol to be used in PCR for lowering melting temperature
On Wed, Sep 14, 2011 at 10:36 PM, <[email protected]> wrote: > Send Methods mailing list submissions to > [email protected] > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/methods > or, via email, send a message with subject or body 'help' to > [email protected] > > You can reach the person managing the list at > [email protected] > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Methods digest..." > > > Today's Topics: > > 1. Re: formamide for DNA (chovek69) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 13 Sep 2011 01:20:57 -0700 (PDT) > From: chovek69 <[email protected]> > Subject: Re: formamide for DNA > To: [email protected] > Message-ID: > <4c300175-5e76-49a6-ad39-318203eaf...@d26g2000yqa.googlegroups.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Try 1,5 Pentanediol... it has huge effect on DNA melting (can lower Tm > with more than 10C) and is absolutely non-toxic. > > > On Aug 19, 8:36 pm, Hiranya Roychowdhury <[email protected]> wrote: >> Sodium chloride ... (can be toxic in high enough concentrations, though ;)) >> >> Hiranya S. Roychowdhury, Ph.D. >> Associate Professor >> Health & Public Services >> NMSU-Dona Ana Community College >> 575 527 7725 (office) > > >> >> ________________________________________ >> From: [email protected] >> [[email protected]] on behalf of Ma,Jin >> [[email protected]] >> Sent: Friday, August 19, 2011 10:13 AM >> To: '[email protected]' >> Subject: formamide for DNA >> >> Dear Sir./Madam, >> Do you know any other non or lower toxic chemicals which can attfect the Tm >> of DNA? >> Thanks. >> Jin Ma >> _______________________________________________ >> Methods mailing list >> [email protected]http://www.bio.net/biomail/listinfo/methods > > > > ------------------------------ > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > > End of Methods Digest, Vol 76, Issue 2 > ************************************** > -- Dr V K Gupta Sr Microbiologist (Mol Biology) IMBL, Department of Entomology Pun. Agric. Univ., Ludhiana (Pb)-141004- India M: 081465-55515 _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
