Sudheer, Medicine smell ?? Medicines smell differently. Can you describe the smell better? Did the colour of the media change after you autoclaved it? And how long did you autoclave it?
Jay -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of sudheer sangeetham Sent: Thursday, September 22, 2011 12:12 PM To: [email protected] Subject: Sterilised LB media getting some medicine smell, while I was growing E.coli culture Hi Guys I am curious to know one thing that, I sterilised the LB media (which was little more diluted). I inoculated the cultures with suitable antibiotics and while I was measuring the OD of bacteria I could smell some medicine was there from the media, I really dont understand why it was like that, but not like earlier. After the OD was 0.55, i induced with IPTG but unfortunately i could nt able to express the protein. The only mistake I did was LB media was little diluted, except this, I think I have done everything right... With curiosity, I would like to know what could be the problem? If any one has any idea...... Thanks in advance -- Sudheer Babu.S Ph.D student Institute of Biochemistry Biological Research Center Szeged,Hungary. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
