Thanks a bunch DK, your suggestions have always helped.
On Tue, Oct 18, 2011 at 10:34 PM, <[email protected]>wrote: > Send Methods mailing list submissions to > [email protected] > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/methods > or, via email, send a message with subject or body 'help' to > [email protected] > > You can reach the person managing the list at > [email protected] > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Methods digest..." > > > Today's Topics: > > 1. Re: Storing NADPH (DK) (DK) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 17 Oct 2011 00:28:26 GMT > From: [email protected] (DK) > Subject: Re: Storing NADPH (DK) > To: [email protected] > Message-ID: <[email protected]> > > In article <[email protected]>, Ygn < > [email protected]> wrote: > >Hi DK, > >To clear my confusion, > > > >I simply take distiled or deionised water, add NaOH to it to take the pH > to > >8.0 and use that for dissolving NADPH? Without any buffer? > > > >What was the "100 mM solution it to" for? I didn't quite get it.. > >Thanks for your suggestion. > > You want to make 100 mM solution that has slightly alkaline pH. > Most nucleotides as sold are acidic when dissolved in water and > their pH needs to be adjusted with NaOH, LiOH or KOH to ~ 8.0. > But now that I see that NADPH is sold as tetrasodium salt, > it seems that its PH when dissolved in water should already be > alkaline. > > DK > > > > > > > ------------------------------ > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > > End of Methods Digest, Vol 77, Issue 7 > ************************************** > -- -- Yoginee Budhkar _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
