On Feb 15, 3:56 pm, Duncan Clark <[email protected]> wrote: > Historians believe that in newspost > <26192644.3453.1329291940527.JavaMail.geo-discussion-forums@vbmh19> on > Tue, 14 Feb 2012, Bjorn <[email protected]> penned the following > literary masterpiece: > > >I am looking for an E. coli strain with a lysogenic lambda phage that is > >practical to induce and purify, such as one with cI857 Sam7. > > >If you have one, I would be most grateful if you could send it to me. > >Alternatively, point me to a resource that is free or not too expensive > >where I could buy one. > > Buy some standard full length lambda cI857 Sam7 methylated DNA from NEB > and electroporate into suitable E.coli host. Screen for lysogen i.e. > grows at 28C but not 42C. > > Or buy in same DNA but find someone with a lambda packing kit, package, > plate screen etc. > > Duncan > -- > I love deadlines. I especially like the whooshing noise they make as > they go flying by. > > Duncan Clark > GeneSys Ltd.
Thanks a lot! but how do I select for transformants? does it have a selection marker? sorry, never worked with lambda... /bjorn _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
