I have had problems with an anti-poly-His Ab (GE cat. no. 27-4710-01) picking 
up something (background) in western blots of HEK 293T cell extracts.  Has 
anyone else had any experience of this?  Is there a better anti-His antibody 
that's popular in the western blotting community?

Thanks,

Paul Phelan
Tufts University Dept. of Biochemistry
Boston

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