Hello I am preparing 10X PBS, 100 ml, the recipe is nacl - 8 g, kcl - 2g, na2hpo4 - 0.14 g , k2hpo4 - 0.027 g. Initially I dissolved these chemicals in 50ml of mq , this time ph was 6.3, when I added 25 ml mq to this, it has shown ph 7.2 I wondered what is happening?
Then I check the 10x sds running buffer ph, it has shown ph 8.65, when I made this to 1x , it has shown 8.0. Then I thought problem with my mq water, then i check the ph of it, it has shown ph 5.6. As I remember the mq water ph 7 or 6.8. I read some of the forums, I came to know that H2o reacts with co2 in the air and forms carbonic acid, may be because of this my mq water ph 5.6. Could any body tell me any soultion? How to degas co2 from mq water? Thank you in advance. -- Sudheer Babu.S Ph.D student Institute of Biochemistry Biological Research Center Szeged,Hungary. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
