Depends. The ones that seal on top can take full volume. The ones that do not and is open should not exceed 75% of the stated volume. Jay
-----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of divya teja Sent: Monday, May 21, 2012 9:22 AM To: [email protected] Subject: regarding volume to be maintained in 12ml ultracentrifuge tubes during ultracentrifugation hello everyone I wanted to isolated rafts from the mouse neuronal cell lines using ultracentrifuge. So, the am having 12ml centrifuge tubes that to be used for ultracentrifugation, but my sample volume itself is 12ml exactly. So, my doubts are: 1. can i use 12ml centrifuge tubes for my 12ml sample????? 2. will there be any spillage of sample???????? 3. Actually what are the minimum and maximum volumes to be maintained in 12ml ultra centrifuge tubes????????? Can you please help me, in clearing my doubts?. thank you regards divya _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
