Hi Divya, why don't you simply split your 12 ml into two times 6 ml? You would need a counterbalance for the centrifuge anyway. Cheers, Niko
> -----Original Message----- > From: [email protected] > [mailto:[email protected]] On Behalf Of > [email protected] > Sent: Montag, 21. Mai 2012 18:07 > To: [email protected] > Subject: Methods Digest, Vol 84, Issue 12 > > Send Methods mailing list submissions to > [email protected] > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/methods > or, via email, send a message with subject or body 'help' to > [email protected] > > You can reach the person managing the list at > [email protected] > > When replying, please edit your Subject line so it is more > specific than "Re: Contents of Methods digest..." > > > Today's Topics: > > 1. regarding volume to be maintained in 12ml ultracentrifuge > tubes during ultracentrifugation (divya teja) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 21 May 2012 15:22:26 +0200 > From: divya teja <[email protected]> > Subject: regarding volume to be maintained in 12ml ultracentrifuge > tubes during ultracentrifugation > To: [email protected] > Message-ID: > > <ca+ck389jsa8cupe0zh-1bwy-j1cfusork-88pqc8af1fjk9...@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > hello everyone > > I wanted to isolated rafts from the mouse neuronal cell > lines using ultracentrifuge. > So, the am having 12ml centrifuge tubes that to be used for > ultracentrifugation, but my sample volume itself is 12ml exactly. > > So, my doubts are: > 1. can i use 12ml centrifuge tubes for my 12ml sample????? > 2. will there be any spillage of sample???????? > 3. Actually what are the minimum and maximum volumes to > be maintained in 12ml ultra centrifuge tubes????????? > > > Can you please help me, in clearing my doubts?. > > > thank you > > > > regards > divya > > > ------------------------------ > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > > End of Methods Digest, Vol 84, Issue 12 > *************************************** > This message and any attachment are intended solely for the addressee and may > contain confidential information. If you have received this message in error, > please send it back to me, and immediately delete it. Please do not use, > copy or disclose the information contained in this message or in any > attachment. Any views or opinions expressed by the author of this email do > not necessarily reflect the views of the University of Nottingham. This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
