You could try the freezer trick to attempt to crystallize and decant, but it sounds like you need a vacuum line or freeze dryer. Your house vacuum for the rotary evaporator is much weaker than the vacuums used for freeze drying and a schlenk line.
Best, Josh On Jun 23, 2012 3:12 PM, "Dr Omar Hamarsheh" <[email protected]> wrote: > Dear Colleagues, > > we are trying to evaporate DMSO from plant extract before being used for > purification in silica gel column. we tried to use rotary evaporator at 65 > C for 3 hours, but seems residual DMSO still existed. colleagues suggest to > put the sample in freezer for 2 two days and them material > will precipitate. > I'll be so grateful for new ideas to remove DMSO. > > thanks in advance, > > omar hamarsheh, PhD > > -- > ------------------------------------- > Omar Hamarsheh, MSc., PhD > Al-Quds University > Department of Biological Sciences > P.O.Box 51000 > Jerusalem > Palestine > > Tel: 00970-599197097 > Fax: 00970-22796960 > > Email: [email protected] > mail.alquds.edu/~f3706/ > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
