In article <[email protected]>, [email protected] says... > *I followed > exactly similar conditions but I could able to detect 60ng of protein. > Actually I am looking for most sensitive method to detect protein on PAGE. > I tried manual Silver staining method(Blue et al) , some times it is > working sometimes not.
In most staining methods staining intensity varies with amino acid composition of the protein in question, seeing 10 ng bands with one and 60 ng with another protein is therefore not surprising. If CBB is not sensitive enough, and silver to finicky, then staining with RuBPS (dye hard) may be the answer: simple, cheap and very sensitive. I have discussed several variations of this method in a recent paper (Meth. Mol. Biol. 869 (2012) 543-550 DOI=10.1007/978-1-61779-821-4_48) _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
