If they were antibiotic resistance plates, they are probably useless and full of satellite colonies. Jay
-----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of David Liu Sent: Monday, August 06, 2012 7:39 PM To: [email protected] Subject: Overgrown (3day) LB plates ok to use? I left a replica LB Amp plate in the 37C over the weekend, and I'd like to know if they're still good to use. I've started liquid cultures but if they grow, can I use the minipreps without resequencing the whole plasmid? It's a replica plate, so I don't necessarily need single colonies, and satellite colonies are not an issue. I am primarily concerned about spontaneous mutations that may have accumulated. Any ideas? _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
