Dear colleagues, I want to raise an antibody against a 5 amino acid motif from a protein sequence.
Form previous experience with small molecules (chemicals) conjugated to KLH I thought that just adding an "innocent" AA (like Ala or Gly) at each of the termini in order to represent the in-sequence character of the peptide and / or also to provide some functional groups for coupling (Cys, Lys) should do the trick. But our contractor suggests a 10-15mer. If necessary, one could do affinity purification against the 5mer afterwards. They say that they do not have experience with such short sequences (but they did antibodies against a chemical for us before). "Our" 5mer sequence arose from mapping a working antibody, i.e. it is a characterized epitope from the protein that we want to detect. The 15AA approach of course makes sense when you have run a protein sequence through some algorithms that try to predict immunogenicity and when you don't have much clues about what will be the real antigen later. Any ideas / suggestions? Thanks for your help! Wo _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
