Hi everyone After elute the protein from Ni-NTA beads I washed the beads with 0.5M NaOH to get rid off imadozole and traces of protein on the bead, but unfortunately the column turns yellow colour? My recombinant protein which fusioned to m-cherry, even after elution the column looks light pink in colour. could anyone explain why it has happened and how I could get back Ni-NTA beads to normal light blue color.
Thank you in advance -- Sudheer Babu.S Research Fellow Institute of Biochemistry Biological Research Center Szeged,Hungary. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
