Hi, I was wondering that will radioactivity incorporation amount remain constant in a pcr reaction over 1 or two half lives?
The basis being that P32 decays into S32 with time, and the question is whether this S32-dNTP will be a substrate for PCR? Because if it is not, can you keep on adding more hot dNTP (to an extent) and freshly prepared probes will be equally hot even after say one or two half lives? so is it Ok to use aP32 labelled probes uptill few half lives of the dntp as long as you are freshly preparing them and compensating amounts in PCR for decay? thanks in advance. Vishal _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
