Interesting question. Looking back, we never used any purified xylene isomer, I think. It was always a solvent grade xylene. for cleaning or as solvent for lipids/waxes. This came as a mixture or all isomers (xylenes?). On the other hand, I think that any pure isomer will also work. The mixture has a higher flashpoint and is safer (still stored in hazardous chem cupboards).
Hiranya S. Roychowdhury, Ph.D. Associate Professor Health & Public Services NMSU-Dona Ana Community College 575 527 7725 (office) ________________________________________ From: [email protected] [[email protected]] on behalf of Gerchman [[email protected]] Sent: Sunday, February 17, 2013 9:25 AM To: [email protected] Subject: FFPE tissue rehydration - which xylene? Greetings all We are trying to rehydrate FFPE samples for protein extraction (I know it is tricky) and the first on the protocol call for wash with Xylene to remove the paraffin. The problem is I am not clear about which xylene as there are at least 3 different isomers (orto, meta and para). Anyone knows which one is used? Many thanks Yoram _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
