Hi I need to isolate DNA from methotrexate resistant cell lines from mouse fibroblasts and do PCR for checking gene amplification. I want to order DHFR (dihydrofolate reductase) primer for amplifying genomic DNA and not RNA. How can I design primer specific for genomic DNA for mouse and where can I do so? also if any one can help me with the DNA isolation protocol as I have not yet started with isolation.
Thanks, Sonal. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
