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Hi Yvonne,
I don't know what to suggest about removing the rust without further
compromising the surface, unless you wanted to re-enamel over the
surface after rust removal? There will be commercial products for this
sort of thing, most likely targeted towards oven repair.

I can imagine how Rust would increase the chances of contamination,
both by providing a ready source of Iron (often limiting to biofilms)
and by providing a rough surface to protect contaminants from exposure
to sprays etc..

I can also imagine this being a common problem; I used to work in a
lab that sprayed everything with strong bleaching agents, which (I am
lead to understand) can lead to pitting of stainless steel over time,
making rust more and more likely. They also used copper sulphate as a
biocide in the water tray of the incubators, which was a good idea,
but the tray was also steel. I'm not sure why it didn't ruin the tray,
but copper sulphate will usually react very quickly with
iron-containing surfaces, which would pit them even faster. If someone
had the "bright idea" of using copper biocides to spray the inside of
your incubator, that would help explain the extreme corrosion.

Best,
Cathal

On 03/11/2013 08:07 PM, Yvonne Couch wrote:
> Hi all, So I am in the process of moving into a new lab. As
> everyone does I come in with pre-conceived ideas about how to do
> things and obviously everyone does things differently but I am
> genuinely concerned about their CO2 incubator. It is almost
> ENTIRELY rust on the inside. Its very smooth so there's no flaking
> but I'm concerned that it's so rusty. My questions are as follows: 
> a) is rust bad in a cell culture incubator? I assume yes but I've
> genuinely never seen it before so I couldn't really say... b) how
> would you get rid of it without making the incubator permanently
> toxic? There is no second incubator running with the same gases at
> the moment so the rust would have to be cleaned a shelf at a time
> so anything that lingered on the shelves might affect the cells. 
> Any thoughts? Y.
> 
> _______________________________________________ Methods mailing
> list [email protected] 
> http://www.bio.net/biomail/listinfo/methods
> 

- -- 
Please note my new email: [email protected]
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