I agree. That was a myth that might have been created by careless grad students :)
Hiranya S. Roychowdhury, Ph.D. Associate Professor Health & Public Services NMSU-Dona Ana Community College 575 527 7725 (office) ________________________________________ From: [email protected] [[email protected]] on behalf of DK [[email protected]] Sent: Thursday, March 14, 2013 11:02 PM To: [email protected] Subject: RE: LiCl precipitation? In article <[email protected]>, "Siefker, Ed B." <[email protected]> wrote: >Sorry about the confusion! I was trying to purify plasmid DNA. >These plasmids are 15K long and don't elute well from typical >miniprep columns. So I have to do it the old fashioned way. Just FYI: that's a myth. I used to routinely purify plasmids > 150 kbp and they elute fine from standard silica columns with a hot (60-70C) 1 mM Tris pH 8.5. DK _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
