Sudheer Sangeetham <[email protected]> wrote: >I was trying to prepare 5x sds sample buffer and it has become brown color >instead of blue color.
Two possibilities: Bromophenol blue is virtually insoluble in water. If the concentration is too high, it stays reddish brown. Try to dilute it with water to 1x and it should turn blue. Then it can be a effect of the pH, since bromophenol blue is a pH indicator as well. Christian -- X-no-Sig: yes _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
