hello everyone, I wanted to isolate plasma membrane from the neuronal cells using PERCOLL (sigma,osmolality max.25 mOsm/kg H2O, density 1.130+/- 0.005).
I need 30% final working percoll solution to isolate plasma membrane.So, in the product information sheet about percol said like we should mix percoll and required media in 9:1 ration and then use the below said given a formula, to get the final working percoll solution. Volume Calculation V0 = V [( r – 0.1)(r10 – 0.9)]/ r0 – 1 V0 = Volume of undiluted Percoll/Percoll PLUS required in ml V = Volume of final working solution in ml r = Desired density of final working solution r0 = Density of Percoll/Percoll PLUS undiluted r10 = Density of 1.5 M NaCl (1.058 g/ml) or 2.5 M sucrose (1.316 g/ml) So, can anybody, please help me to solve my problem like from 9:1 percol mix, how much volume should I take to get 30% final working percoll solution and what will be the density of my 30% percoll working solution. Thank you in advance for your time and consideration. waiting for your replies. best regards, Divya _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
