I have a quick question for everyone: When using glutathione Sepharose (4B Fast Flow from GE) to purify a GST-tagged protein, has anyone used it at pH 6.5 and have it work? I ask because I am trying to purify a DNA-binding protein that is greatly aggregated after the initial glutathione Sepharose column, and I think it might be pH-related. However, I do think the elution of the fusion protein from glutathione Sepharose will still have to be at pH 8.0 for it to work.
Thank you, Paul Phelan Tufts University Boston _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
