Additionally, You may add DNase(in order to manufacture protocol) On 4/28/15, Fulvio Celsi <fulvio.ce...@gmail.com> wrote: > when they are so viscous is due to DNA...try to make a milder lysis..or you > can spin them @14K and take the supernatant ..or also sonicate it... > > Fulvio Celsi > BsC,PhD > Immunobiology Lab. > *I.R.C.C.S. materno infantile Burlo Garofolo* > via dell'Istria, 65/1 > 34137 > Trieste > Italy > Office +390403785273 > Mobile: +393286489131 > > 2015-04-28 13:53 GMT+02:00 Alejandro Miguel Martin Dunn < > alejandro.mar...@cigb.edu.cu>: > >> Plain vortexing has worked for me in the past. I have also tried >> squirting >> the sample through a small bore needle (gauge 23) using an insulin >> syringe, >> and it works, but is messier. >> >> Hope it helps! >> >> -----Original Message----- >> From: methods-boun...@oat.bio.indiana.edu [mailto: >> methods-boun...@oat.bio.indiana.edu] On Behalf Of Phelan, Paul J. >> Sent: Monday, April 27, 2015 2:45 PM >> To: meth...@magpie.bio.indiana.edu >> Subject: Whole cell extract gel samples for western blot >> >> Sometimes our gel samples (whole cell extracts) for western blots are >> very >> viscous. What are the best methods for lysing small cell extracts well >> enough so that they don't float back out of the wells when loaded? >> >> Thank you, >> >> Paul Phelan >> Tufts University Boston >> _______________________________________________ >> Methods mailing list >> Methods@net.bio.net >> http://www.bio.net/biomail/listinfo/methods >> >> >> _______________________________________________ >> Methods mailing list >> Methods@net.bio.net >> http://www.bio.net/biomail/listinfo/methods >> > _______________________________________________ > Methods mailing list > Methods@net.bio.net > http://www.bio.net/biomail/listinfo/methods >
-- Anna Feldman, Phd, Laboratory of Computational Systems Biology (Tuller lab), Biomedical Dept, Faculty of engineering, TAU annafeldm...@gmail.com 03-6407582 0547606327 _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
