You can use the A260/A280 as an indicator of protein purity. Ideally, pure
protein should have an A260/A280 value of 0.6, so 1.05 is very high, and
indicates that you have nucleic acid contamination (the A260 component is too
high).
________________________________________
From: methods-boun...@oat.bio.indiana.edu [methods-boun...@oat.bio.indiana.edu]
on behalf of Sudheer Sangeetham [sudheer.pb...@gmail.com]
Sent: Wednesday, October 28, 2015 7:13 AM
To: meth...@oat.bio.indiana.edu
Subject: the absorbance value 1.05 at 260/280 of protein solution
Hello people
What does it mean by the absorbance value 1.05 at 260/280 while measuring
the protein concentration at 280 nm ?
Thank you in advance
--
Sudheer Babu.S
Research Fellow
Institute of Biochemistry
Biological Research Center
Szeged,Hungary.
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