Hello everyone, I would glad if someone can give me an hint about my trouble... I would like to transfect my cell line with a plasmid GFP-Protein of interest to overexpress it. Transfection should be transient and so, after a few generation I should lose my overexpressed protein in several cells. In any case I should select GFP population after transfection with FACS and sorting, but on these cells I would like to do mitotic spreads... So I have to put the positive cells in plate for colcemid... Could you give me an hint? If I select a population GFP positive, how can I do later to get a good number of cells for a rapid colcemid treatment in time to mantain a good number of GFP cells so as not to miss plasmid and protein, but so as I could do a good number of spreads? I hope I was clear... Thank you anyone... Pasquale _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
