It's not very easy but if you have the equipment is achievable. See here: https://www.researchgate.net/publication/14362548_Rapid_DNA_Sequencing_of_More_Than_1000_Bases_per_Run_by_Capillary_Electrophoresis_Using_Replaceable_Linear_Polyacrylamide_Solutions
and improvement with DMSO here: http://www.google.com/patents/WO2002055662A2?cl=en I use Beckman's gel syringes, but with in-house prepared electrophoresis buffer (TTE). You can combine the leftovers from several syringes by connecting them with cut p200 pipette tip and pushing. Bubbles are not a problem ! Good luck _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
