On Monday, April 21, 2003 at 6:30:10 AM UTC-7, Subhash Kulkarni wrote: > you are shaking the molten media too much before u plate it, avoid doing > that, just keep the molten media calm and then pour with agitation, that > should do the trick, no gas is being LIBERATED > > Michael Witty wrote: > > On 20 Apr 2003, bob wrote: > > > > > >>I put my agar plates in 37 degress celsius, I take them out and I > >>have little air bubbles/pockets embedded in my agar. I beleive some > >>gas is being release by the agar and trapped in the agar. The media I > >>use is LB. I don't know if it was Lennox broth or Luriel broth. LB > >>is LB to me. My bacterial still grows but the air bubbles makes things > >>a little harder to see and they also don't look very nice. > >>This occurs occasionally. What gas is being released and what can I > >>do to fix it? > > > > > > . . . happened to me intermittantly as well. Don't know what caused it. > > Don't know what made it stop. Yes, it is annoying! Regards, Mike. > >
I did the exactly same thing that Subhash Kulkarni mentioned. That really causes the trapped air bubbles inside of the LB-Agar Plates. _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
