Does the protein size match the cut-off of the concentrator? How do you measure protein concentration?
On 27.12.18 03:01, m.golgolj...@gmail.com wrote:
Hi, I'm in cas9 purification with the help of ni-nta agarose I use amicone 100 for further purification After using the amicone, my protein concentration decreases I do not know why I use 500 mg of imidazole for purification Please guide me Thank you _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
_______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
