Dear MISO users, I am trying to run MISO on my TopHat (version 2.0.14) files, but couldn’t get any results. It kept returning the following message:
Loading BAM filename from: input.sorted.bam Filtered out 1 read pairs that were on same strand. Filtered out 1 reads that had no paired mate. - Total read pairs: 0 No. reads discarded due to strand violation: 0 Only 0 reads in gene, skipping (needed >= 20 reads) Computing Psi for 1 genes... - 6:144385735:144385588|144385605:-@6:144290044:144290115:- - GFF filename: index/miso/hg19/chr6/6:144385735:144385588|144385605:-@6:144290044:144290115:-.pickle - BAM: input.sorted.bam - Outputting to: MISOtest - Paired-end mode: (200.0, 15.0) It appears that MISO couldn’t extract reads from my bam file. Could someone help? Thanks, Woody _______________________________________________ miso-users mailing list [email protected] http://mailman.mit.edu/mailman/listinfo/miso-users
