----- Forwarded message from Taylor Luneau -----

Date: Thu, 15 Nov 2012 16:35:40 -0500
From: Taylor Luneau
Reply-To: Taylor Luneau
Subject: help
To: [email protected]

I've been using the tps analysis programs to compare growths rates of Landlocked Atlantic
Salmon at Vermont Hatcheries in comparison to Vermont rivers and have
recently run into a few problems. I was hoping that you could help me
with some of the details and maybe offer advice on the best way to
conduct some statistical tests using this software.Ive
 assembled a large data set of pictures of atlantic salmon, sampled at Ed weed hatchery, Eisenhower national fish hatchery as well as the Huntington
river. We sampled the two hatcheries over a series of months in 2011
and 2012. What i am hoping to do is compare the fish at the two
hatcheries and look for differences in growth based off of shape and
size. I would also like to compare the average shapes and sizes of
each sample group to the Huntington fish (our "wild" fish and
therefore our control).

     To do this I have taken pictures of each fish, (12 per sample/ 3
samples at each hatchery/ 1 sample at huntington river) and used the
analysis programs tpsutil and tpsdig2 to place landmarks on each fish,
at specific locations, to generate the average shape of each fish and
how it differs from the general population.

   I was wondering if you had any thoughts about how i could run these
geometric morphometric analysis tests most efficiently or perhaps if
you knew anyone who does and would be willing to help me? Overall, i
would like to identify the geometric perimeters characteristic of a
smolt (A smolt is a juvenile salmon which has developed to the point
where it begins to migrate to the ocean)  so as to provide hatchery
workers with the general shape and size of a smolt ready for release
into the watershed. What would you suggest to be the easiest and
quickest ways to do this? Centroid size? Principle component analysis?
Do i need both? All your help is greatly appreciated and i look
forward to speaking with you more soon.

Taylor


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