Hello everyone

I am currently working on a morphometric analysis of the head shape in a
invertabrate - planarians. Given that these are soft-bodied organisms with
few homologous anatomical structures, I have found that fixed landmarks
alone are insufficient to accurately capture the complexity of their
morphology.

I am interested in implementing sliding semilandmarks to better represent
the curves and outlines of the head region. However, I am seeking guidance
on the best practices for this specific case, particularly regarding:

   1.

   The optimal spacing and density of semilandmarks for highly plastic
   shapes.
   2.

   Software recommendations or R packages (e.g., geomorph) that handle
   sliding semilandmarks effectively for outlines without many fixed anchor
   points.
   3.

   Any specific protocols for minimizing measurement error in organisms
   that lack rigid biological anchors.

If anyone has experience applying these methods to soft-bodied
invertebrates or can point me toward relevant literature, I would greatly
appreciate your insights.

Thank you in advance for your time and assistance.

Best regards,

Carlos

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